Syed Abdul Qadir Shah
Fraunhofer Institute for Biomedical Engineering
Cell Biology & Applied Virology
Ensheimer Str. 48
66386 St. Ingbert, Germany
Tel.: +49 (0) 6894 980 186
Fax.: +49 (0) 6894 980 185
Sensitive chip-based assays to investigate physical, chemical and biological factors regarding their influence on the cell biological effects of nanoparticles (Project 3).
Related work Packages: WP1 and WP4 (see WP Table).
Abdul obtained his bachelor degree in microbiology in 2008 from Quaid-e-Azam University Islamabad, Pakistan. He joined National Reference Lab for Poultry Diseases in NARC Islamabad as a research assistant where he worked on the isolation of avian influenza virus by serological testing of blood samples. After graduation he pursued his master degree in molecular biology at University of Skövde, in Sweden. He did his master thesis work at High Tech Campus Philips Eindhoven in Netherland. He defended his master thesis with the title “Optimization of bacterial cell lysis on-bench and on-chip” in June 2010. In May 2011 he joined Fraunhofer Institute of Biomedical Engineering St. Ingbert Germany to start his Marie Curie fellowship in NanoTOES project.
NNanotechnology has turned into widespread commercialization which results in potential health risks of nanoparticles. Existing standardized in vitro toxicological assays average the response of a complete cell population; thus ignores single-cell response. Conventional methods for single cell analysis often use trypsinized cells which compromise physiological conditions. Therefore new technologies are required for verifying the toxicity of nanoscale materials non-invasive on single cell level.
Currently the lab-on-chip systems are widely applied in cell-based assays for toxicology and drug analysis. This project will focus on cell-based chip technologies for non-invasive characterization of cells in vitro. The project aims to develop sensitive chip-based assays to investigate physical, chemical and biological factors regarding their influence on the cell biological effects of nanoparticles. A microchip-based lensless imaging system will be optimized and used for this purpose. The system allows us to assess continuously the cell behavior (proliferation, attachment on the surface, morphology) by direct projection of shadow images. The assays developed on microchip-based lensless imaging system would be validated by comparing with existing standardized toxicity assays.
Prof. Dr. Hagen von Briesen (NanoTOES PI, Fraunhofer), Dr. Yvonne Kohl (Fraunhofer)