Health Effects Laboratory
Norwegian Institute for Air Research
Instituttveien 19, 2007 Kjeller, Norway
Tel: (+47) 6389 8168
Assessment of genotoxic potential of nanoparticles, oxidative DNA damage and repair (Project 6)
Related Work Packages: WP1, WP2, WP4, WP5 (see WP Table).
Anna Huk obtained her Master of Sciences Engineered degree in Environmental Protection Technology in 2010 from Gdansk University of Technology (Poland), where she worked on combine bioremediation and bio-fumigation process by using plant form Brasica family (AGROBIOKAP project – www.chem.pg.gda.pl/agrobiokap). She completed it in October 2010, defending her thesis entitled “The impact of cultivation condition on the content of bioactive substances with the biofumigation potential in Brassica oleracea var Capitata f. alba”. In 2010 she got scholarship at Norwegian Institute for Air Research in Health Effects Laboratory, where she worked on optimization of 12 gels format for the Comet assay to increase efficiency in nanogenotoxicology study (part of NanoTEST project – www.nanotest-fp7.eu). In 2011, after successful selection from almost 100 applicants, she came back to Norway to start her Marie Curie Fellowship within the NanoTOES project in the Health Effects Group.
- Kusznierewicz B, Bączek-Kwinta R, Bartoszek A, Piekarska A, Huk A, Manikowska A, Antonkiewicz J, Namieśnik J, Konieczka P. The dose-dependent influence of zinc and cadmium contamination of soil on their uptake and glucosinolate content in white cabbage (Brassica oleracea var. capitata f. alba). Environ Toxicol Chem. 2012; 31(11):2482-9.
- Hudecová A, Kusznierewicz B, Hašplová K, Huk A, Magdolenová Z, Miadoková E, Gálová E, Dušinská M. Gentiana asclepiadea exerts antioxidant activity and enhances DNA repair of hydrogen peroxide- and silver nanoparticles-induced DNA damage, Food Chem Toxicol. 2012; 50 (9):3352-9.
Nanotechnology is one of the rapidly developing fields of technology which products can find application in every brunch of industry, from cosmetics to new methods of preservative food, but of course, the biggest attention is putting now to use of nanoparticles in nanomedicine. However, every new technology, especially those which will be used in medicine, should be strictly examined before they come to patients or customer use. In our research we focus on genotoxic potential of nanoparticles as some may cause DNA damage. If this damage is not repaired it may result in mutagenicity. Mutations in genetic material of somatic cells can lead to the development of diseases such as cancer. In case the DNA damage occurs in reproductive cells it may have impact on health and quality of life of next generation. In our project we are using in vitro human and mammalian cells to detect DNA strand breaks, oxidative DNA damage, gene mutations, chromosomal alterations and clastogenic effects using the comet assay, gene mutation and micronucleus assays additionally to assays detecting cytotoxicity and oxidative stress. The focus is on correlations between the size, shape, coating of nanoparticles and their impact on genome. We also try not only to answer the question “if nanomaterials are dangerous” but also to define the mechanism of their toxicity at different molecular and cellular levels.
Because of huge variation in nanotoxicolgy studies and discrepancies in the results, we also aim to increase the quality of nanotoxicology studies by establishing standard operating procedures for in vitro toxicology laboratory according to Good Laboratory Practices. We also are looking on possible problems, such as the interference between nanomaterials and standard genotoxic assays. The aim is also to modify assays for toxicity testing of nanomaterials and to develop high throughput methods for more efficient and robust toxicity testing.
Prof. Albert Duschl Ph.D (NanoTOES PI, ICN), RNDr. Maria Dusinska Ph.D. (NanoTOES PI, NILU), Alessandra Rinna, Ph.D. (NILU).