Institute for Work and Health (IST)
Rte de la Corniche 2
CH-1066 Epalinges – Lausanne, Switzerland
Tel: (+41) 21 314 74 63
Influence of low-volatile organic pollutants on the intrinsic ROS-production capacity (Project 7)
Related Work Packages: WP1, WP2, WP3, WP4 (see WP Table).
Jiayuan obtained her bachelor degree majored in environmental engineering from the China University of Mining and Technology, Beijing in 2009. After that, she pursued and obtained her master at Clarkson University in America under Professor Philip Hopke. During this period, she was involved as the major contributor or a joint researcher in several projects focusing on air pollutants (reactive oxygen species, volatile organic compounds, carbonyls…) characterization in different circumstances. Also, she gained experience with receptor modeling. In 2011, Jiayuan accepted the offer to work as a PhD student in the frame of the Marie-Curie training programme NanoTOES at the Institute for Work and Health and University of Lausanne Switzerland.
- J. Zhao, P.K. Hopke, Concentration of reactive oxygen species (ROS) in mainstream and sidestream cigarette smoke. Aerosol Sci. Technol. 46:191–197 (2012).
- T.R. McAuley, P.K. Hopke, J. Zhao, S. Babaian, Comparison of the Effects of E-cigarette Vapor and Cigarette Smoke on Indoor Air Quality. Inhalation Toxicology (in press, 2012).
A better understanding of the capacity of nanoparticles (NP) to produce reactive oxygen species (ROS) could contribute to the understanding of the NP toxicity and human health. Cell-free fluorescent measurement of the ROS production has the advantages of being feasible, stable and sensitive. We were able to verify different approaches using 2’7-dichlorodihydrofluorescin (DCFH) to detect ROS generation and have provided a reliable acellular protocol that takes into consideration key issues to conduct a successful experiment. Moreover, this protocol has been applied to characterized brake wear samples’ oxidative reactivity along with other analysis.
Compared to coarse and fine particles, NPs are known to have a much larger surface area which has been suspected to relate to their toxicity. Our topic will contribute to clarifying the surface function effects of NPs. Low-volatile organic compounds (LVOC) with a high affinity to surfaces will be coated on NPs in a home-made dynamic system. The change of NPs’ ROS capacity will be studied along with other techniques to detect the coating using various microscopy techniques. We expect different results by applying different LVOCs: some may contribute to redox-cycling to increase oxidative reactivity while some may block the reactive sites on NPs surface to decrease ROS capacity.
We are also looking forward to collaborating with other partners to further study with cell-based systems the possible health effects induced by the LVOC coating on NP surface.
PD Dr.sc.nat. Michael Riediker (NanoTOES PI, IST)