GRIMM Aerosol Technik GmbH & Co. KG
Telephone: (+43) 662 8044 5884 (University of Salzburg)
Linda obtained her inter-university Bachelor degree in Molecular Life Sciences in 2009 from the University of Salzburg (Austria) and the Johannes-Kepler-University in Linz (Austria). After graduation, she pursued her Masters in Molecular Biology at the University of Salzburg and joined the group of Prof. Dr. Albert Duschl in April 2010 where she performed the experimental work for her Master thesis. She completed her Masters in April 2011, with her thesis entitled “Effects of silver nanowires on human bronchial epithelial cells”. Since May 2011 she is working as a Marie Curie Fellow within the NanoTOES project and is enrolled in the Molecular Biology doctoral study program of the University of Salzburg.
- Stoehr L.C., Gonzalez E., Stampfl A., Casals E., Duschl A., Puntes V., Oostingh G.J.: Shape matters: effects of silver nanospheres and wires on human alveolar epithelial cells. Particle & Fibre Toxicology 2011, 8: 36.
Cell-based tests are an important and frequently used tool for assessing the biological impact of nanoparticles, but most of them require cell culture facilities and specially trained personnel. Focusing on airborne nanoparticles, which easily change their properties during sampling, Linda’s project aims at the development of biological assays for on-site application, which will allow a more realistic exposure model and maintain the physico-chemical properties of the nanoparticles. One part of the study focuses on different ways to store cells on-site and to culture them under conditions expected during on-site measurements. This includes both traditional cell culture as well as air-exposed culturing using specific inserts for cell culture plates, in order to mimic real life conditions.
In a second part, the cells are exposed to different aerosols of known size distribution, concentration and composition under controlled conditions and the influence of particles contaminated with different compounds, such as the endotoxin lipopolysaccharide (LPS), chemicals that are often adsorbed to airborne particles (polycyclic aromatic hydrocarbons (PAHs), sulfates, nitrates etc.) or allergens, will be tested. Finally, another part deals with the assessment and optimization of adequate particle deposition onto cells, which is one of the key factors for realistic and representative particle exposure.
Dr. Markus Pesch (GRIMM Aerosol Technik GmbH & Co. KG), Prof. Dr. Albert Duschl (PLUS) and Dr. Gertie Janneke Oostingh (University of Applied Sciences in Salzburg)