University of Salzburg
5020 Salzburg, Austria
Tel: (+43) 662 8044 5884
New cellular and cell-free models for assessing NP-induced oxidative stress (Project 1)
Related Work Packages: WP1, WP2, WP3, WP4 (see WP Table).
After completing the International Baccalaureate at Bonn International School in 2004, Paul obtained his Bachelor degree (B.Sc. Hon.) in Medical Biochemistry in 2008 from the University of Glasgow. He then studied at Edinburgh Napier University to obtain his Master (M.Sc.) in Biomedical Science at the end of 2009, where he investigated the possible adverse effects of silver nanoparticles on the male reproductive system. Following his Masters, Paul moved back to Germany and worked at the University of Bonn before he started his Marie Curie Fellowship as an early stage researcher (PhD student) at the University of Salzburg.
- Haas B., Schlinkert P., Mayer P., Eckstein N., Targeting adipose tissue, Diabetol. Metab. Syndr. 4 (1), (2012).
Many reports suggest that oxidative stress associated with production of reactive oxygen species (ROS) is induced by NP. Paul will perform a study to screen for particularly relevant gene regulation events related to cellular stress, and will go on to produce a cell line with a relevant promoter, e.g. hemoxygenase-1. The effect of silver NP, known to be immunotoxic, on different lung epithelial cells will be compared to gold NP using real time PCR, Western blot, ELISA and flow cytometry analysis. The main focus during this study will be on the potential of NP to induce oxidative stress via the production of ROS.
In addition, cell-free tests developed by partner 7 will be used to analyse the ability of NP to trigger the formation of ROS in the absence of cells. The balance between cellular antioxidants and ROS (generated both on the surface of particles and by cells following exposure to NP) will determine the induction of cellular stress. The project will thus investigate both intrinsic and cell-mediated mechanisms leading to cellular stress responses.
Main skills acquired by Paul during this project are molecular and cell-based assays, and their application to investigating nanomaterial. As part of this project Paul will undergo secondments to other NanoTOES partners (ICN, IST, GRIMM) which will contribute towards his research.
Prof. Dr. Albert Duschl (NanoTOES PI, PLUS)