Project 11 (Grimm)
Project 11: Biological assays for on-site application
Fellow: Linda Stöhr (ESR10), 36 months
Tutor: Dr. Hans Grimm (Grimm)
Cell-based assays are important tools for assessing nanoparticle effects on cells since they are relatively quick and cheap, do not require experimental use of animals and can be adapted for medium-to-high throughput formats. However, their application is at present mostly confined to research laboratories since institutions (mostly companies) manufacturing or using nanomaterials are usually lacking personnel and facilities for cell culture and cell based tests. Within this translational project, an ESR fellow will adapt and apply a laboratory-based test for on-site tests towards biological effects of nanoparticles.
The ESR will make use of reporter cell tests established at PLUS (see project 1) which will be used on-site at a manufacturing location. In order to perform on-site experiments under realistic conditions, two different problems will have to be solved. First, it has to be established how cells can be stored, transported and prepared for use outside of a biological laboratory. This part of the project will be performed at and assisted by PLUS, making use of different methods for cooling and freezing combined for storage and regular vs. air-liquid surface cell culture setups. Second, cells need on site to be exposed to air in controlled conditions regarding airflow, volume etc. This part will be performed at Grimm, which is only about 10 km from PLUS, so one person can perform both tasks. It is crucial that one fellow performs the work both within and outside a dedicated biological laboratory, since the translation of laboratory methods to real life situation requires equal skills in both environments.
The exposure experiments will compare normal working conditions with simulated accidents resulting in high exposure to nanoparticles. In further experiments, contaminants (LPS, volatile chemicals, toxins, allergens etc.) will be intentionally added to the airflow in realistic concentrations in order to analyse the effect of mixture toxicities where nanoparticles are one of the compounds. This project relies on the capabilities of PLUS for cell-based assays and on the competence of the SME Grimm in assessing airborne nanoparticles. The fellow will acquire skills both in cell-based and physical methods for NP analysis, and in practical transfer of a method from the laboratory to a manufacturer developing a product. Secondments to partners 1 (cell assays), 2 (endotoxin assays), 10 (method development under GLP).
In WP1 (task 1.5) and WP4 (task 4.5).